Pip2a-mcherry

Author: ygpl

August undefined, 2024

Webb17 mars 2016 · Roots or leaves of 2-week-old A. thaliana plants expressing PIP2A-mCherry were inoculated with Ct-GFP spores (2–5 × 10 5 /ml) and imaged by confocal microscopy after 1 to 14 days. Plant Growth Promotion Assay. Webb17 okt. 2024 · Moreover, the green fluorescence of GmALMT5-GFP was co-localized with the red fluorescence from PIP2A-mCherry, which adds further support to the conclusion that GmALMT5 was localized on the plasma membrane (Figure 4). To further confirm these results, transient expression of GmALMT5-GFP was also assayed in onion epidermal cells.

Root Endophyte Colletotrichum tofieldiae Confers Plant Fitness

WebbThe free GFP fluorescence signal did not colocalize with either the FM4-64 or the PIP2A-mCherry fluorescence, confirming the expected nuclear and cytoplasmic localization of … Webb27 juli 2024 · The fusion construct 35S::FaPKc2.2-eGFP and the plasma membrane (PIP2A-mCherry) or the cytosol marker (RPP3A-mCherry) were co-expressed in the … henley fred perry

Plant Immunity NAIST-BS

WebbPanels (A,C) are the image of leaf epidermis and elongated root cells, respectively, of 5 days old plant showing Rab: GFP signal in green, PIP2A: mCherry signal in red, merge of … Webb5 okt. 2024 · The potential plasma membrane marker PIP2A–mCherry was used as a full-length protein and fused C-terminally to GFP . Analysis of the marker in leaf epidermal … Webb9 nov. 2024 · PIP2A protein was verified to be located on the plasma membrane at the cellular level (Cutler et al. 2000). The recombinant plasmids pBI121-eGFP, pBI121-eGFP … henley freemasons

Maize YSL2 is required for iron distribution and development in …

Webb28 jan. 2024 · (a) Co-localization of P3a and PIP2A-mCherry. PIP2A acts as a PM marker; ( b ) Co-localization of P3a and its interacting proteins. AtPUP14-CFP, AtGTR1-CFP, and … WebbmCherry ﬂuorescence disappeared from epidermal cells soon Figure 2. C. toﬁeldiae Colonization of A. thaliana Roots Confocal microscope images of C. toﬁeldiae (Ct) expressing cytoplasmic GFP (green) and A. thaliana expressing PIP2A-mCherry (magenta). (A) Spores (s) germinating on a leaf form melanized appressoria (arrows); 8 dpi. Overlay ... henley free peopleWebb12 juni 2013 · Copper (Cu) homeostasis in plants is maintained by at least two mechanisms: (1) the miRNA-dependent reallocation of intracellular Cu among major Cu-enzymes and important energy-related functions; (2) the regulation of the expression of Cu transporters including members of the CTR/COPT family. These events are controlled by … large outdoor clock and thermometer set

"Webb3 feb. 2024 · Constitutively expressed mCherry (proUBQ10::PIP2A-mCherry) was the screenable marker used to identify transformed seeds. Sequences of the T-DNAs … " - Pip2a-mcherry

Pip2a-mcherry

PIP2A plasma membrane intrinsic protein 2A [ (thale cress)]

Webb6 okt. 2024 · Representative confocal fluorescent microscopy images of double transgenic plants expressing Pro35S:GFP-RabA2b and membrane marker aquaporin PIP2a-mCherry. Panels (A,C) are the image of leaf epidermis and elongated root cells, respectively, of 5 days old plant showing Rab: GFP signal in green, PIP2A: mCherry signal in red, merge of … WebbPIP2;1 is an integral membrane protein that facilitates water transport across plasma membranes. To address the dynamics of Arabidopsis thaliana PIP2;1 at the single …

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Webb15 maj 2014 · Transient co-expression of the GFP constructs with the tonoplast marker γ-Tonoplast Intrinsic Protein (TIP)-mCherry or the plasma membrane marker Plasma membrane Intrinsic Protein 2A (PIP2A)-mCherry and the pBIN-NA construct in leaf abaxial epidermal cells of Nicotiana tabacum was performed via the agroinfiltration procedure … Webb7 apr. 2016 · (L and M) Intracellular hyphae inside a root cortical cell (c) enveloped by PIP2A-mCherry-labeled host membranes (arrows); 8 dpi. Maximum projections of z stack images. (M) Enlargement of (L) using a subset of optical sections. Scale bars, 20 …

WebbPubMed Webb13 apr. 2024 · (A) Confocal microscopy of epidermis of Nicotianna benthamiana transiently expressing 35S:NILR1-GFP and plasma membrane marker 35S:PIP2A-mCherry. Scale, …

WebbThe species P. cucumerina is well known as a plant pathogenic fungus that naturally infects A. thaliana and has been deeply studied as model for necrotrophic interactions … WebbAAV is usually considered BSL-1, but may require BSL-259 handling depending on the insert. Storage. Store at -80°C. Thaw just before use and keep on ice. Quality Control. …

Webb11 okt. 2011 · In the root cells co-expressing mCherry-tagged VAMP721 and fluorescence marker of Golgi, mCherry-VAMP721-labeled organelles were in physical proximity with …

Webb10 aug. 2024 · We found that sfGFP, Venus, mCherry and mCherry2 all caused substantial foci formation, despite being monomers or very weak dimers when expressed alone. mKate2 and TagRFP-T caused … large outdoor bows christmasWebb28 jan. 2024 · (a) Co-localization of P3a and PIP2A-mCherry. PIP2A acts as a PM marker; ( b ) Co-localization of P3a and its interacting proteins. AtPUP14-CFP, AtGTR1-CFP, and AtNRT1.7-CFP were co-expressed with P3a-RFP in N. benthamiana leaves; ( c ) Subcellular localizations of AtPUP14-CFP, AtGTR1-CFP, and AtNRT1.7-CFP during BrYV infection; … henley frc shirtsWebb13 nov. 2024 · Subcellular localization markers fused to mCherry include the nuclear marker NLS-mCherry, the endoplasmic reticulum (ER) marker HDEL-mCherry, the Golgi … henley freegleWebb27 okt. 2024 · We further fused this NLS fragment and mCherry to the N- and C-termini of PIP2A, a protein marker for the plasma membrane, respectively, and expressed the protein in tobacco leaves . Our … henley fours headWebb29 mars 2024 · Confocal microscope images of Colletotrichum tofieldiae expressing cytoplasmic GFP (green) and Arabidopsis thaliana expressing PIP2A-mCherry … henley freecycleWebb22 aug. 2024 · Stomatal movements are crucial for the control of plant water status and protection against pathogens. Assays on epidermal peels revealed that, similar to … henley four oaks caravan club siteWebb2 juni 2024 · GFP and mCherry emission signals were detected with HyD (hybrid) detectors ranging from 500 to 530 nm and 580 to 650 nm, respectively. In planta overexpression of … large outdoor christmas star decoration