WebJan 6, 2012 · The original gpIs1 transgene (pCL25, Figure 1) was created using a plasmid that contained the promoter region of the hsp-16.2 gene fused to the coding sequence of GFP and the 3′untranslated region (UTR) from unc-54, which was empirically used to drive expression of transgenes . This construct was manually injected into hermaphrodite … WebHere we determine if the ability to predict life span and thermotolerance is specific to the gpIs1 insertion or a general property of hsp-16.2-based reporters. New single-copy hsp-16.2-based reporters predict life span and thermotolerance. We conclude that prediction power of hsp-16.2-based transcriptional reporters is not an artifact of any ...
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