Genome analyses of single human oocytes

Author: pxhn

August undefined, 2024

Web6 hours ago · Using third-generation nanopore sequencing technology, the researchers have characterized mtDNA heteroplasmy in single cells and described the genetic features of … WebApr 23, 2024 · To the best of our knowledge, this is the first study to use multi-omics single-cell analysis to compare the transcriptomes, DNA methylomes and genomic CNVs of IVT and IVV matured human oocytes. Single-cell analyses of differences in the transcriptomic profiles of oocytes derived from women with and without PCOS and at different stages of ...

Genome Analyses of Single Human Oocytes - LWW

Web13 rows · Dec 19, 2013 · Here, we demonstrate genome analyses of single human oocytes using multiple annealing and ... WebAug 30, 2024 · Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation. ... 41 against build hg38 of the human ... csproj none link

Study finds mitochondrial mutations in single cells

WebJan 30, 2014 · Single-cell analyses of human embryos following in vitro fertilization (IVF) ... Hou Y, Fan W, Yan L, Li R, Lian Y, et al. (2013) Genome analyses of single human oocytes. Cell 155: 1492–1506. View Article Google Scholar 46. Conrad DF, Keebler JE, DePristo MA, Lindsay SJ, Zhang Y, et al. (2011) Variation in genome-wide mutation … Web20 hours ago · New knowledge about mitochondrial DNA (mtDNA) mutations has been obtained through the use of a high-throughput single-cell, single-mitochondrial genome sequencing technique known as iMiGseq. This technology also provides a platform for evaluating mtDNA editing techniques and genetically diagnosing embryos before … WebMay 22, 2013 · Pathway Analysis of Single Human Oocytes and Blastocysts. We analysed components of 10 different pathways that were highly represented (P<0.05; q<0.22) in our individual human oocyte and … csproj migrate

Human embryonic genome activation initiates at the one-cell …

Genome analyses of single human oocytes Yu Hou, Ph.D.

WebDec 19, 2013 · In 2013, Tang and Qiao's group [139] used single-cell WGS to reveal the morphological and dynamic changes of chromosomes during oocyte development, and … Web33 minutes ago · An international team of researchers, led by KAUST stem cell biologist Mo Li, has now quantitatively depicted the genetic maps of mtDNA in single human oocytes (immature eggs) and blastoids (stem cell-based synthetic embryos). [1] This has revealed molecular features of rare mtDNA mutations that cause maternally inherited diseases. csproj post build eventsWebAug 30, 2024 · Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation. ... dj riri

"WebDec 21, 2024 · Summary. In human embryos, the initiation of transcription (embryonic genome activation [EGA]) occurs by the eight-cell stage, but its exact timing and profile are unclear. To address this, we profiled gene expression at depth in human metaphase II oocytes and bipronuclear (2PN) one-cell embryos. High-resolution single-cell RNA … " - Genome analyses of single human oocytes

Genome analyses of single human oocytes

Study finds mitochondrial mutations in single cells

WebSep 2, 2024 · Developing female human germ cells undergo genome-wide epigenetic reprogramming, but de novo DNA methylation dynamics and their interplay with chromatin states and transcriptional activation in developing oocytes is poorly understood. Here, we developed a single-cell multi-omics sequencing method, s … WebGenome Analyses of Single Human Oocytes. Author links open overlay panel Yu Hou 1 6, Wei Fan 1 4 6, Liying Yan 1 6, Rong Li 1, Ying Lian 1, Jin Huang 1, Jinsen Li 1, Liya …

Did you know?

WebApr 13, 2024 · A human blastocyst-like synthetic embryo called blastoid showing the presence of an enveloping layer of extra-embryonic cells, a blastocoel-like cavity, …

WebJie Qiao and colleagues describe how high-throughput sequencing of oocyte polar bodies could enhance preimplantation genomic screening in IVF. WebHere, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing …

WebIn single human sperm cells, MALBAC accurately detects chromosomal abnormalities and DNA sequence variations associated with genetic disorders but has not been evaluated … WebApr 6, 2024 · Background Oocyte maturation arrest and early embryonic arrest are important reproductive phenotypes resulting in female infertility and cause the recurrent failure of assisted reproductive technology (ART). However, the genetic etiologies of these female infertility-related phenotypes are poorly understood. Previous studies have mainly …

WebSingle-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening. However, the nonuniformity of single-cell whole …

WebJan 8, 2024 · A total of 149 human oocytes were collected at the Centre for Reproductive and Genetic Health (CRGH), London (UK), after obtaining appropriate research consent. The oocytes were collected between the years 2015 and 2024. The oocytes were donated by 42 women between the ages of 25 and 44 years (mean ± SD of maternal age 35 ± 3.4 … dj rinkWeb19 hours ago · New knowledge about mitochondrial DNA mtDNA mutations has been obtained through the use of a high-throughput single-cell single-mitochondrial genome sequenci. ... The genetic maps of mtDNA in individual human oocytes (immature eggs) and blastoids (synthetic embryos derived from stem cells) have recently been quantitatively … dj rio nashvilleWebApr 14, 2024 · Dolosigranulum pigrum phylogenetic and core genome analysis. We first analyzed the genetic diversity of 21 D. pigrum whole genome sequences available (n = 7 from NCBI and n = 14 from in-house D ... dj rip love faouzia versi koploWebGenome Analyses of Single Human Oocytes. Author links open overlay panel Yu Hou 1 6, Wei Fan 1 4 6, Liying Yan 1 6, Rong Li 1, Ying Lian 1, Jin Huang 1, Jinsen Li 1, Liya Xu 1, Fuchou Tang 1 5, X. Sunney Xie 1 2, Jie Qiao 1 3. ... Whole-genome amplification and sequencing of single human oocytes using MALBAC method ... dj rio de janeiroWeb18 hours ago · For example, oocyte quality, embryonic development, and fertility can be restored by nicotinamide mononucleotide treatment in aged mice . Germline nuclear transfer, including germinal vesicle, pronuclear, and spindle transfer, is a potential strategy for improving the meiotic competence of aged mouse and human oocytes (93, 94). csproj none updateWebApr 4, 2024 · Unbiased analysis of the whole mutational landscape of mtDNA in single human oocytes. (A) ... csproj outdirWeb33 minutes ago · Using third-generation nanopore sequencing technology, the researchers have characterized mtDNA heteroplasmy in single cells and described the genetic … csproj post build step